Everything Is Faster: How Do Land-Grant University-Based Plant Diagnostic Laboratories Keep Up with a Rapidly Changing World?

Plant diagnostic laboratories (PDLs) are at the heart of land-grant universities (LGUs) and their extension mission to connect citizens with research-based information. Although research and technological advances have led to many modern methods and technologies in plant pathology diagnostics, the pace of adopting those methods into services at PDLs has many complexities we aim to explore in this review. We seek to identify current challenges in plant disease diagnostics, as well as diagnosticians' and administrators'perceptions of PDLs' many roles. Surveys of diagnosticians and administrators were conducted to understand the current climate on these topics. We hope this article reaches researchers developing diagnostic methods with modern and new technologies to foster a better understanding of PDL diagnosticians' perspective on method implementation. Ultimately, increasing researchers' awareness of the factors influencing method adoption by PDLs encourages support, collaboration, and partnerships to advance plant diagnostics.

Characterization, Virulence, Epidemiology, and Management of Anthracnose in Celery.

Leaf curling and petiole twisting of celery (Apium graveolens) were observed in several commercial fields in five Michigan counties in 2010 through 2012, causing significant crop damage and loss. Prior to this time, the pathogen Colletotrichum acutatum species complex had not been previously associated with celery in Michigan. In this study, the pathogen's genotype and phenotype were characterized, the influence of environmental conditions determined, and fungicides tested. Pathogen identification was based on conidial morphology and molecular identification using species-specific primers. Intersimple-sequence repeat (ISSR) banding patterns were similar between C. acutatum isolates from celery (n = 51) and blueberry (n = 1) but different from C. dematium and C. gloeosporioides. Four ISSR primers resulted in 4% polymorphism when tested on isolates from celery. Pathogenicity and virulence of C. acutatum sensu lato isolated from celery (n = 81), tomato (n = 2), and blueberry (n = 1) were evaluated in greenhouse experiments, which revealed differences in virulence among isolates but no significant differences specific to collection year, county, or field. In dew chambers and growth chambers, high temperatures (≥25°C) or long leaf wetness duration (>24 h) increased disease incidence. Twelve fungicides were tested in field studies over two growing seasons to determine their efficacy against celery anthracnose. The fungicides azoxystrobin, pyraclostrobin, mancozeb, and chlorothalonil reduced disease by 27 to 50% compared with the untreated control when disease pressure was moderate.